fefab|The antiretroviral activity of APOBEC3 is inhibited by the foamy。,wnbr 2022

The molecular clone of feline foamy virus (pCF-7) and FeFAB cells for FeFAB assay were kind gifts from Dr Martin Löchelt, German Cancer Research Center, Germany.

Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plafefabsmids encoding either wt or mutant Bet (Figure。

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